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Direct-zol RNA Microprep Kits

Highlights
Easy Handling: Bypass chloroform, phase separation and precipitation steps.
NGS-Ready: Ultra-pure RNA without phenol carryover. No DNA contamination (DNase I included).
Non-Biased: Complete RNA recovery without miRNA loss.

서한형 대리

Zymo Research 제품 담당자

경신과학(주)

영업부

H.P) 010-8832-6303

HanHyung Seo

Zymo Research Brand Manager

Kyongshin scientific Co., Ltd.

Sales Department

H.P) 82)10-8832-6303

제품소개

Highlights

Easy Handling: Bypass chloroform, phase separation and precipitation steps.
NGS-Ready: Ultra-pure RNA without phenol carryover. No DNA contamination (DNase I included).
Non-Biased: Complete RNA recovery without miRNA loss.

Description

The Direct-zol RNA MicroPrep Kits are RNA purification kits that provide a streamlined method for the purification of up to 10 µg (per prep) of high-quality RNA directly from samples in TRI Reagent® or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.). Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol™ method assures unbiased recovery of small RNAs including miRNA. The procedure is easy. Simply apply a prepared sample in TRI Reagent® directly to the Zymo-Spin™ Column and then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The eluted RNA is high quality and suitable for subsequent molecular manipulation and analysis (including RT-PCR, transcription profiling, hybridization, sequencing etc.). The entire procedure typically takes only 7 minutes.

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Performance

Technical Specifications

Compatibility	TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®.
Equipment	Microcentrifuge, vortex
Sample Inactivation	TRI Reagent® (provided with R2061, R2063) inhibits RNase activity and inactivates viruses and other infectious agents.
Sample Source	Any sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)).
Size Range	Total RNA ≥ 17 nt
Yield	10 µg RNA (binding capacity), ≥6 µl (elution volume)

Resources

Documents

Protocol:

Datasheet:

SDS (MSDS):

SDS (MSDS): TRI Reagent

Supplemental Info.

FAQ

Q1: Is Direct-zol suitable for very small numbers of cells?

Yes, the Direct-zol MicroPrep (#R2060) is designed and capable of purifying RNA down to single cell inputs (picogram amounts). A sensitive quantification method is needed (e.g. Qubit, qPCR, etc.)

Q2: Is DNase I available for individual purchase?

All kit components are available for purchase separately.

Q3: How to store DNase-I following resuspension?

Lyophilized DNase I is stable at room temperature. Once resuspended, store frozen aliquots. Minimize freeze thaw cycles as much as possible. Freeze thaw will lower DNase activity.

Q4: Is the DNase-I treatment necessary?

If the downstream application requires DNA-free RNA, we recommend performing the DNase I treatment.

Q5: Is the kit compatible with samples stored in DNA/RNA Shield?

Yes, bring samples homogenized and stored in DNA/RNA Shield to room temperature (20-30ºC). Add an equal volume (1:1) of TRIzol/TRI Reagent and mix well. Proceed with RNA Purification.

Q6: Is it possible to extract proteins with the Direct-zol RNA kits?

Yes, proteins can be acetone precipitated from the column flowthrough. Please see the Protein Purification appendix in the protocol.

Q7: I ran out of RNA Wash Buffer. Can I use something else?

Yes, use 80% ethanol as a substitute. RNA Wash Buffer is also sold separately.

Q8: Can samples be stored in TRIzol/TRI Reagent prior to processing?

Yes, samples in TRIzol/TRI Reagent or similar are stable overnight at room temperature and can be stored frozen (-80C). Be sure to lyse and homogenize the sample well prior to freezing. Bring the sample to room temperature prior to RNA Purification.

Q9: Is it possible to isolate DNA with the Direct-zol RNA kits?

Direct-zol DNA/RNA (R2080) kits can isolate DNA from TRIzol

Q10: Is the RNA suitable for Next-Gen sequencing or other sensitive downstream applications?

Yes, the RNA is high quality (A260/A280 >1.8, A260/A230 >1.8) and suitable for any downstream application, including NGS, RT-PCR, hybridization, etc.

Q11: Which phenol-based reagents are compatible with Direct-zol?

The Direct-zol kits are compatible with TRI Reagent, TRIzol, Qiazol, RNAzol, TriPure, TriSure, etc., and any other acid-guanidinium phenol-based reagents.

Q12: What is the difference between the Direct-zol RNA and Quick-RNA kits?

Direct-zol is for samples stored/collected into TRIzol/similar reagents. Quick-RNA is for all other samples.

Q13: What is the difference between the Direct-zol RNA MiniPrep and the Direct-zol RNA MiniPrep Plus?

Both kits function the same, the only difference is the RNA binding capacity of the column provided with the kit.

Q14: What is the average RNA yield? (chart)

Input	Average RNA Yield
Cells	10 µg (per 106 cells)
HeLa	15 µg
High Yield Tissue (mouse)	≥ 30 µg (per 10 mg)
Spleen	30-50 µg
Liver	40-60 µg
Low Yield Tissue (mouse)	≤ 30 µg (per 10 mg)
Brain, Heart	5-15 µg
Muscle	5-20 µg
Lung	10-20 µg
Intestine	10-30 µg
Kidney	20-30 µg
Whole Blood	(per 1 ml)
Porcine	10-20 µg
Human	2-10 µg

Q15: How to improve purity, RIN scores and eliminate contamination (i.e., A260/230, A260/280 ratios, DNA, phenol, protein, salt, etc.)?

Purity, RIN and/or any type of contamination can result from initial sample preparation (i.e., inefficient lysis of the sample). To improve, increase the volume of the lysis reagent (e.g., TRI Reagent/TRIzol or RNA Lysis Buffer).

Q16: Is this kit compatible with CTAB-based RNA extraction methods?

Yes, the Direct-zol RNA kits are compatible with CTAB-based RNA extraction methods for polysaccharide-rich and/or phenolics-rich samples (e.g., Pinus, Geranium plants). Please find detailed protocol here.

Reviews

"Before I discovered this kit, I was isolating RNA the old school way with chloroform and it would take half the day to finish the protocol. The Direct-zol RNA Miniprep kit is AWESOME! It took hardly any time, the protocol was so easy, and my RNA quality was SO much better. Honestly, this kit revolutionized my life at the bench."

A. Newhart

The Wistar Institute

"Simple protocol and yielded good quality of RNA. Only one kit working for all type of tissue, cell and especially biological fluids."

Mohan K.

University of Illinois, Chicago

"Previously I used a protocol that took 3 hours, now I can have my RNA in 20 minutes. What is not to like about that? Just one column and two buffers, I love it."

Arjan V.

Indiana University

주문정보

CAT.No	품명	규격
R2060	Direct-zol RNA Microprep	50 preps
R2061	Direct-zol RNA Microprep (Product Supplied w/ 50 ml TRI Reagent)	50 preps
R2062	Direct-zol RNA Microprep	200 preps
R2063	Direct-zol RNA Microprep (Product Supplied w/ 200 ml TRI Reagent)	200 preps