ENGLISHHOMECONTACT USSITEMAP
Zymo Research
▪ HOME>제품정보>Zymo Research
Quick-RNA MagBead
Highlights
Versatile: High-throughput, magnetic bead-based isolation total RNA (including small/micro RNAs) from any sample including cells, solid tissue, whole blood, biological liquids, FFPE tissue, environmental (plant/seed), swabs (stool, soil, microbial samples), etc.
NGS-Ready: High-quality RNA is ready for any downstream application. DNase I included.
서한형 대리

Zymo Research 제품 담당자

경신과학(주)

영업부

H.P) 010-8832-6303

HanHyung Seo

Zymo Research Brand Manager

Kyongshin scientific Co., Ltd.

Sales Department

H.P) 82)10-8832-6303

제품소개

Highlights

  • Versatile: High-throughput, magnetic bead-based isolation total RNA (including small/micro RNAs) from any sample including cells, solid tissue, whole blood, biological liquids, FFPE tissue, environmental (plant/seed), swabs (stool, soil, microbial samples), etc.
  • NGS-Ready: High-quality RNA is ready for any downstream application. DNase I included.

Description

The Quick-RNA MagBead kit provides a high-throughput, magnetic bead-based purification of high-quality total RNA (including small/microRNAs) from any sample source (e.g., cells, solid tissue, whole blood, biological fluids, FFPE tissue, environmental (plant/seed), swabs (stool, soil, microbial samples)), samples stored in DNA/RNA Shield, etc. The provided DNA/RNA Shield inactivates infectious agents and is ideal for sample storage at ambient temperatures. Total RNA is eluted into ≥50 µl of DNase/RNase-Free Water and is ready for any downstream application including Next-Gen Sequencing, RT/PCR, hybridization, etc.

For automation scripts and support, email automation@zymoresearch.com

Performance

Technical Specifications
Binding Capacity15 µg total RNA per 30 µl MagBinding Beads.
Equipment NeededMagnetic stand or separator, heat block, liquid handler or robotic sample processor (user provided).
PurityHigh-quality RNA is ready for Next-Gen Sequencing, RT/PCR, hybridization, etc.
Recommended Materials(available separately) – 96-well Collection Plate (C2002; capacity is up to 1.2 ml/well), 96-Well Block (P1001; capacity is up to 2 ml/well), 96-well Elution Plate (C2003), Cover Foil (C2007), ZR-96 MagStand (P1005).
Sample PreservationDNA/RNA Shield lyses cells, inactivates nucleases and infectious agents and is ideal for safe sample storage and transport at ambient temperatures (page 7).
Sample SourcesAny cells, solid tissue, whole blood, biological fluids, FFPE tissue, environmental (plant/seed), swabs (stool, soil, microbial samples), samples stored in DNA/RNA Shield, etc.
Size LimitsTotal RNA including small/microRNAs ≥ 17nt.
StorageRNA eluted with DNase/RNase-Free Water (provided) can be stored frozen. The addition of RNase inhibitors is highly recommended for prolonged storage.

Resources

Protocol: 
Datasheet: 
SDS (MSDS): 

FAQ

InputAverage RNA Yield
Cells10 µg (per 106 cells)
HeLa15 µg
High Yield Tissue (mouse)≥ 30 µg (per 10 mg)
Spleen30-50 µg
Liver40-60 µg
Low Yield Tissue (mouse)≤ 30 µg (per 10 mg)
Brain, Heart5-15 µg
Muscle5-20 µg
Lung10-20 µg
Intestine10-30 µg
Kidney20-30 µg
Whole Blood(per 1 ml)
Porcine10-20 µg
Human2-10 µg

Purity, RIN and/or any type of contamination can result from initial sample preparation (i.e., inefficient lysis of the sample). To improve, increase the volume of the lysis reagent (e.g., TRI Reagent/TRIzol or RNA Lysis Buffer).

  • To ensure complete lysis, increase the volume of the lysis reagent (i.e., increase or titrate the volume of TRI Reagent/TRIzol or RNA Lysis Buffer). Lysate should be clear (not opaque or viscous). Pellet debris by centrifugation (if needed) and process the cleared supernatant.
  • Prior to adding TRI Reagent/TRIzol or RNA Lysis Buffer, perform enzymatic treatment (Proteinase K; #D3001-2-20) and/or mechanical homogenization (bead beating with ZR BashingBead Lysis Tubes; #S6003) in DNA/RNA Shield (#R1100).

This can happen on occasion due to transport or storage at lower temperatures. The reagent functionality is not affected; however, the precipitate can be resolved by heating the reagent to >37 °C.

주문정보

CAT.No 품명 규격 비고
R2132 Quick-RNA MagBead 96 preps
R2133 Quick-RNA MagBead 4 x 96 preps