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Zymo Research
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OneStep PCR Inhibitor Removal Kit
▶ Fast, one step procedure for cleaning impure samples prior to PCR, sequencing, NGS, reverse transcription (RT), etc.


▶ Efficiently eliminate polyphenolics from extracted DNA & RNA samples for PCR and short or long read sequencing.


▶ ≥80% recovery of PCR inhibitor free DNA & RNA.
서한형 대리

Zymo Research 제품 담당자

경신과학(주)

영업부

H.P) 010-8832-6303

HanHyung Seo

Zymo Research Brand Manager

Kyongshin scientific Co., Ltd.

Sales Department

H.P) 82)10-8832-6303

제품소개





Product Description


The OneStep PCR Inhibitor Removal kits are PCR inhibitor clean up kits that contain all the components needed for efficient removal of contaminants from DNA and RNA preparations that can inhibit downstream enzymatic reactions (e.g. PCR and RT). The column slurry has been optimized to efficiently bind and remove any polyphenolic compounds, such as humic/fulvic acids, tannins, melanin, etc. from the most impure DNA and RNA preparations. Sample clean-up is as simple as filtering your sample with the prepped column.

Learn More

Technical Specifications

Applicable forCleaned samples are ready for PCR, sequencing, reverse transcription (RT), etc
Input Volume50-200µl
EquipmentMicrocentrifuge
PurityHigh quality, enzymatic-reaction ready DNA/RNA products.
Sample sourceSamples contaminated by polyphenolics that inhibit enzymatic reactions.
Sample storageEluted DNA/RNA can be used immediately or stored at ≤ - 20°C.
Typeds/ssDNA and RNA.
Yield80-90%

Resources


Q1: What is the minimum and maximum input volume that can be added to the OneStep Inhibitor removal kit?

The minimum volume that can be processed with the HRC columns is 50µl. For the maximum volume, the columns can handle as much as 200µl, however, the input limit is mostly dependent on the amount of inhibitors present in the sample. For largely inhibited samples it is recommended to keep volumes between 50-100µl.

Q2: What is the difference between the old OneStep PCR Inhibitor Removal Kit (D6030) and this new version of the kit (D6031)?

The new version has a more simplified workflow as it does not require the addition of any prep buffer and has improved stability for more efficient inhibitor removal.

Q3: How do I access the old format?

The old format (D6030) will be available until July 2025. You can access the old format by clicking here.

주문정보

CAT.No 품명 규격 비고
D6031 OneStep PCR Inhibitor Removal Kit (NEW) 50 Preps
D6035 OneStep-96 PCR Inhibitor Removal Kit 2 x 96 Preps