Zymo Research는 1994년도에 설립된 미국 회사로 고품질 키트 제조회사 입니다.
각종 샘플(세포, 조직, 환경샘플 등)로부터 고품질
DNA나 RNA를 가장 쉽고 빠르게 뽑을 수 있습니다.
이 외에도, 각종 Epigenetics 관련 제품들
(DNA Methylation kit 등)과 Microbiomics
(샘플 채집부터 분석까지의 전 단계의 제품)
연관 제품들이 준비되어 있습니다.
Universal Depletion: Novel probe-free technology depletes rRNA from any organism.
Simplest Kit: Prepare NGS-grade RiboFree cDNA from total RNA in as little as 1.75 hours.
Automation Friendly: Streamlined protocol for increased scalability.
서한형 대리
Zymo Research 제품 담당자
경신과학(주)
영업부
H.P) 010-8832-6303
HanHyung Seo
Zymo Research Brand Manager
Kyongshin scientific Co., Ltd.
Sales Department
H.P) 82)10-8832-6303
제품소개
HIGHLIGHTS
Universal Depletion: Novel probe-free technology depletes rRNA from any organism.
Simplest Kit: Prepare NGS-grade RiboFree cDNA from total RNA in as little as 1.75 hours.
Automation Friendly: Streamlined protocol for increased scalability.
DESCRIPTION
The Zymo-Seq RiboFree Universal cDNA Kit is the simplest kit for the preparation of NGS-grade first-strand cDNA with ribosomal RNA (rRNA) depleted. This simple cDNA kit features RiboFree Universal Depletion, a novel probe-free rRNA depletion technology that depletes rRNA from any organism. The RiboFree Universal Depletion is validated across biological kingdom and phyla, including mammals, plants, and various prokaryotes, and also RNA from a wide range of sample types including cells, snap-frozen tissues, and FFPE samples. The RiboFree single-stranded cDNA produced by the kit can be used for qPCR detection, RNA-Seq library prep, and any other downstream analysis. In contrast to poly(A) enrichment methods, the RiboFree Universal cDNA Kit produces single-stranded cDNA from nearly all RNA biotypes, including full-length mRNA, mRNA with degraded poly(A) tails, long-noncoding RNA (lncRNA), intronic RNA, nucleolar RNA, etc. The cost-effective Zymo-Seq RiboFree Universal cDNA Kit has been optimized to reduce hands-on time, with all the reagents such as SPRI beads included for researchers’ convenience.
Equipment Needed (user provided)
Thermal cycler with heated lid, magnetic stand for 0.2 mL PCR tubes, microcentrifuge for 0.2 mL PCR tubes and 1.5 mL microcentrifuge tubes, and a benchtop vortex mixer.
Sample Input Material
RNA
RNA Input
10 – 1000 ng of total RNA.
For optimal results, please use the recommended 10-1000 ng input.
If an input below 10 ng is necessary, see Appendix B of the kit protocol for additional considerations and recommended modifications.
Input Quality
RNA should be free of DNA contamination and enzymatic inhibitors, with A260/A280 and A260/A230 ≥ 1.8. RNA with lower purity ratios (A260/A280 and A260/A230) should be treated with DNase I and purified with the RNA Clean & Concentrator™ (Cat. No. R1013) prior to processing. RNA should be suspended in water, TE, or a low-salt buffer.
For optimal results, please use intact RNA (RNA Integrity Number or RIN ≥ 8.0) whenever possible.
For degraded RNA input, see Appendix C of the protocol for additional considerations and recommended modifications.
It is possible to prepare NGS-grade cDNA with input lower than 10 ng, though the performance may be negatively impacted. For modifications of the protocol when using 1-9 ng of RNA as input, please refer to Appendix B in the kit protocol for detailed suggestions or contact tech@zymoresearch.com.
There are many ways for you to be certain about which instruction manual to follow. One way is to check the cold reagent names: they are different between the two versions, and the updated version includes a reagent called "Depletion Reagent 4". Overall, compared to v1.1.0, the updated version has a lower minimum input (10 ng total RNA) and a shorter depletion reaction time (1 hour for 100 ng of input). To learn more of the kit features since the update, or if you need an electronic copy of the previous instruction manual (v1.1.0), please contact tech@zymoresearch.com.
