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Quick-DNA/RNA Viral MagBead
Highlights
High-throughput, magnetic-bead based purification of viral DNA and RNA from plasma, serum, urine, cell culture media, blood, saliva, cellular suspensions, swab, fecal and biopsy samples
High-quality DNA/RNA ready for Next-Gen sequencing, RT/qPCR, hybridization, etc.
DNA/RNA Shield included for sample collection, inactivation and storage.
서한형 대리

Zymo Research 제품 담당자

경신과학(주)

영업부

H.P) 010-8832-6303

HanHyung Seo

Zymo Research Brand Manager

Kyongshin scientific Co., Ltd.

Sales Department

H.P) 82)10-8832-6303

제품소개

Highlights

  • High-throughput, magnetic-bead based purification of viral DNA and RNA from plasma, serum, urine, cell culture media, blood, saliva, cellular suspensions, swab, fecal and biopsy samples
  • High-quality DNA/RNA ready for Next-Gen sequencing, RT/qPCR, hybridization, etc.
  • DNA/RNA Shield included for sample collection, inactivation and storage.

Description

Quick-DNA/RNA Viral MagBead kit is designed for high-throughput purification of viral DNA and/or RNA from plasma, serum, urine, cell culture media, blood, saliva, cellular suspensions, biopsies and swab and fecal samples stored in DNA/RNA Shield. The kit also features a buffer system that facilitates complete viral particle lysis for efficient nucleic acid isolation. Small (>50 nt) and large (>200 kb) DNA and RNA are bound to magnetic beads, washed and eluted. The isolated high-quality nucleic acids are ready for all downstream applications such as Next-Gen sequencing, hybridization-based and RT/PCR detection.

Learn more about early viral detection

For automation scripts and support, email automation@zymoresearch.com

Performance

Technical Specifications
EquipmentAutomated liquid handler with heating element, plate shaker, and 96-well magnetic stand (not provided). The procedure can also be performed manually.
PurityRNA is ready for Next-Gen sequencing, RT-qPCR, microarray, hybridization, etc.
Sample SourcePlasma, serum, saliva, urine, blood, cell culture media, cellular suspensions, biopsies and swab and fecal samples
Size Range50 nt to ~200 kb
Yield5 µg DNA/RNA binding capacity (per 10 µl MagBinding Beads), ≥15 µl elution volume.

Resources

Protocol: R2140/R2141 | R2140E/R2141E | R2143
Datasheet: 
SDS (MSDS): EU | US
Automation Notes: 

FAQ

The purpose of beta-mercaptoethanol is to help with deproteination. It is not necessary if you are working with simple samples such as swabs. It is recommended if you are working with protein rich samples such as plasma, serum, blood, saliva, sputum, etc.

Yes, these samples are compatible.

Yes, this kit will co-purify some DNA.

Most downstream application methods for viral detection do not require DNase treatment during purification. If necessary, DNase treatment can be perform post-purification and additional components can be purchased separately (i.e., DNase I, DNA Digestion Buffer, RNA Prep Buffer and RNA Wash Buffer).

Yes, the change from clear to yellow is a result of oxidation and will not affect the performance of the buffer.

The Viral RNA Buffer is guaranteed for one year from the date of purchase even with the addition of beta-mercaptoethanol. Just be sure to close the bottle cap tightly to prevent evaporation.

In most cases, it could be because the samples contain high amounts of protein or cellular debris. To help prevent this in future preps, it’s best to add beta-mercaptoethanol to the Viral RNA buffer, and/or implement a Proteinase K digestion step.

For optimal results and detection of viral target, collect sample in DNA/RNA Shield and perform Proteinase K treatment. In addition, add beta-mercaptoethanol to the Viral RNA Buffer prior to purification, as well as perform all steps at room temperature and centrifugation speeds at 10,000-16,000 x g to ensure no buffer retention.

The “-E" markings at the end of the catalog numbers of “-Dx” products indicate that they are CE-IVD verified for in vitro diagnostic use.

This can happen on occasion due to transport or storage at lower temperatures. The reagent functionality is not affected; however, the precipitate can be resolved by heating the reagent to >37 °C.

주문정보

CAT.No 품명 규격 비고
R2140 Quick-DNA/RNA Viral MagBead 250 Preps
R2141 Quick-DNA/RNA Viral MagBead 1000 Preps
R2140-E Quick-DNA/RNA Viral MagBead – DX 250 Preps
R2141-E Quick-DNA/RNA Viral MagBead – DX 1000 Preps
R2143 Load N’ Go DNA/RNA Viral Kit 96 Preps